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U-2973, a novel B-cell line established from a patient with a mature B-cell leukemia displaying concurrent t(14;18) and MYC translocation to a non-IG gene partner

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Abstract

B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein–Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U-2973 displayed a mature B-cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G-banding, fluorescent in situ hybridization, spectral karyotyping and single nucleotide polymorphism array demonstrated a complex karyotype with both a t(14;18) and double translocations between MYC and a non-IG gene partner located at chromosome 12p12.1.
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Keywords: B-cell; IGH/BCL2; MYC; cell line; leukemia; lymphoma; t(14;18)

Document Type: Research Article

Affiliations: 1: Department of Hematology, Uppsala University Hospital, Uppsala, Sweden 2: Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden 3: Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden

Publication date: September 1, 2008

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