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CD226 is expressed on the megakaryocytic lineage from hematopoietic stem cells/progenitor cells and involved in its polyploidization

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Ma D, Sun Y, Lin D, Wang H, Dai B, Zhang X, Ouyang W, Jian J, Jia W, Xu X, Jin B. CD226 is expressed on the megakaryocytic lineage from hematopoietic stem cells/progenitor cells and involved in its polyploidization.

Eur J Haematol 2005: 74: 228–240. © Blackwell Munksgaard 2005. Abstract: 

Objectives:  In this study, we examined the expression of CD226 on megakaryocytic, granulocytic and erythroid lineage from hematopoietic stem cells/progenitor cells in adult and fetus and its potential role in megakaryocytic maturation. Methods: CD34+ cells from adult and fetus were induced to differentiate toward the megakaryocytic lineage by thrombopoietin (TPO) and the granulocytic lineage by granulocyte colony-stimulating factor (G-CSF), respectively. Mononuclear cells from fetal liver and CD34+ cells from adult were induced to differentiate toward erythroid-lineage by erythropoiesis (EPO). We investigated the expression of CD226 and lymphocyte function associated antigen-1 (LFA-1) (CD11a) during hemopoiesis. We also studied the effect of CD226 monoclonal antibody (MoAb) and LFA-1 MoAb on megakaryocyte with antibody cross-liking technique. Results: CD34+ cells from adult and fetus and TPO-induced CD41+ cells all expressed CD226 molecule. CD226 was not expressed on erythroid progenitor cells and erythroblasts and most cells of granulocytic lineage although G-CSF induced a significant increase of the expression of CD226 on CD34+ cells in early period of time. CD226 MoAb acts on megakaryocytes by inducing intracellular calcium mobilization. The expression of LFA-1 decreased significantly at late stage of differentiation and maturation of fetal megakaryocytes whereas the expression of LFA-1 on adult megakaryocytes retained at a high level. CD226 MoAb in combination with LFA-1 MoAb shifted the ploidy of generated megakaryocytes from adult-derived CD34+ cells to higher classes significantly although CD226 and LFA-1 MoAb slightly increased the ploidy of the generated megakaryocytes individually. CD226 MoAb or LFA-1 MoAb or CD226 MoAb plus LFA-1 MoAbs did not increase the ploidy of the generated megakaryocytes from fetus-derived CD34+ cells. Conclusion: CD226 molecules play an important role in maturation of the megakaryocytes in combination with LFA-1.
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Keywords: CD226; LFA-1; hematopoietic stem cell; megakaryocyte; ploidy

Document Type: Research Article

Affiliations: 1: Department of Experimental Medicine, Northern Hospital, Wenhua Road, Shenyang, Liaoning 2: Department of Immunology, The Fourth Military Medical University, Xi'an, Shaanxi, China

Publication date: March 1, 2005

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