Skip to main content
padlock icon - secure page this page is secure

Correlation of c-kit expression and cell cycle regulation by transforming growth factor-beta in CD34+ CD38 human bone marrow cells

Buy Article:

$59.00 + tax (Refund Policy)

Morita N, Yamamoto M, Tanizawa T. Correlation of c-kit expression and cell cycle regulation by transforming growth factor-beta in CD34+ CD38 human bone marrow cells.

Eur J Haematol 2003: 71: 351–358. © Blackwell Munksgaard 2003. Abstract:

To investigate the relationship between c-kit expression and cell cycle regulation by endogenous transforming growth factor-beta (TGF-β) in human bone marrow hematopoietic progenitor cells, CD34+ CD38c-kitlow/− and CD34+ CD38c-kithigh populations were cultured in stem cell factor, thrombopoietin, interleukin-3 (IL-3), IL-6, granulocyte colony-stimulating factor, granulocyte/macrophage colony-stimulating factor and anti-TGF-β, and analyzed for cell cycle status. Arrest in G0/G1 was most prominent in the precultured CD34+ CD38c-kitlow/− subset (95.62 ± 4.15%). While postcultured CD34+ CD38c-kithigh cells initiated from CD34+ CD38c-kithigh cells entered cell cycle within 36 hr, postcultured CD34+ CD38c-kitlow/− cells initiated from CD34+ CD38c-kitlow/− cells remained dormant until 36 hr and entered cell cycle within 90 hr. Anti-TGF-β increased the percentage of S/G2M phase postcultured CD34+ CD38c-kithigh cells (from 19.08 ± 11.95 to 47.04 ± 2.93%), but no significant change was observed in postcultured CD34+ CD38c-kitlow/− cells. These results suggest that endogenous TGF-β plays an important role in the cell cycle arrest of c-kithigh but not c-kitlow/− cells in CD34+ CD38 cells, which proliferate without undergoing differentiation. The different regulatory mechanism of cell cycle entry of the CD34+ CD38c-kithigh and CD34+ CD38c-kitlow/− subsets might be the result of differences in their sensitivity to endogenous TGF-β.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: G0/G1 arrest; c-kit; cell cycle; endogenous transforming growth factor-beta; human bone marrow hematopoietic progenitor cells

Document Type: Research Article

Publication date: November 1, 2003

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more