Analysis of natural killer (NK) cell activity and adhesion molecules on NK cells from umbilical cord blood
Eur J Haematol 2003: 71: 29–38. © Blackwell Munksgaard 2003. Abstract:
The activity of natural killer (NK) cells in human umbilical cord blood (CB) has been reported to be low, compared with that in adult peripheral blood (PB) in vitro. To examine the cause of this, after dividing the CD56+/CD3− cells in CB and PB into CD56bright and CD56dim NK cells, the NK cell activities and the expression of various surface antigens were assayed for each fraction. The NK cell activity of CD56dim NK cells in CB was significantly lower than that in PB (P = 0.0003), whereas, there was no significant difference between the NK cell activity of CD56bright NK cells in PB and CB. The expression levels of adhesion molecules (CD2, CD11a, CD18, DNAX accessory molecule-1), CD16, and CD57 for CD56dim NK cells in CB were significantly lower than those in PB, and approximately one-third of CB CD56dim NK cells were capable of forming conjugates with K562 cells, compared with PB CD56dim NK cells. Furthermore, the inhibition of both the NK cell activities and binding of CD56dim NK cells in PB and CB by monoclonal antibody against each of these adhesion molecules suggests that they play an important role in NK cell activity. These findings show that the low NK cell activity in CB is caused by the low NK cell activity of CD56dim NK cells and that the low expression level of adhesion molecules on CB CD56dim NK cells may contribute to this low NK cell activity.
Document Type: Research Article
Affiliations: 1: Department of Transfusion Medicine, 2: Division of Hematology and Oncology, Department of Internal Medicine, 3: Laboratory of Cell Transplantation, Institute for Advanced Medical Sciences, 4: Department of Pediatrics, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
Publication date: July 1, 2003