@article {Johansson:2003:0902-4441:358,
title = "The presence of a significant association between elevated PRV-1 mRNA expression and low plasma erythropoietin concentration in essential thrombocythaemia",
journal = "European Journal of Haematology",
parent_itemid = "infobike://mksg/ejh",
publishercode ="bp",
year = "2003",
volume = "70",
number = "6",
publication date ="2003-06-01T00:00:00",
pages = "358-362",
itemtype = "ARTICLE",
issn = "0902-4441",
eissn = "1600-0609",
url = "https://www.ingentaconnect.com/content/mksg/ejh/2003/00000070/00000006/art00003",
doi = "doi:10.1034/j.1600-0609.2003.00079.x",
keyword = "essential thrombocythaemia, myelosuppression, thrombosis, erythropoietin, PRV-1",
author = "Johansson, Peter and Andr{\’e}asson, Bj{\"o}rn and Safai-Kutti, Soodabeh and Wennstr{\"o}m, Lovisa and Palmqvist, Lars and Ricksten, Anne and Lindstedt, G{\"o}ran and Kutti, Jack",
abstract = "Johansson P, Andr{\’e}asson B, Safai-Kutti S, Wennstr{\"o}m L, Palmqvist L, Ricksten A, Lindstedt G, Kutti J. The presence of a significant association between elevated PRV-1 mRNA expression and low plasma erythropoietin concentration in essential thrombocythaemia. Eur J Haematol 2003: 70: 358362. \textcopyright Blackwell Munksgaard 2003. Abstract: Approximately 45% of newly diagnosed patients with essential thrombocythaemia (ET) demonstrate subnormal plasma erythropoietin (EPO) concentrations, which constitutes a risk factor for occlusive vascular events. In 58 ET patients, a possible association between polycythaemia rubra vera-1 (PRV-1) overexpression and subnormal plasma EPO was investigated, which was always measured prior to the institution of platelet lowering agents. At the time when PRV-1 expression was measured, 28 of 58 (48%) ET patients had received platelet lowering treatment. PRV-1 expression was measured by quantitative real-time reverse transcriptionpolymerase chain reaction assay of mRNA extracted from purified peripheral blood buffy coat. The cycle threshold (CT) value of PRV-1 was determined and was divided with the CT value for the housekeeping GAPDH gene transcript. A quotient P=0.001). The 30 ET patients who had not received any platelet lowering treatment showed a significant (P=0.005) relation between PRV-1 positivity and subnormal plasma EPO. No such relationship was present in the 28 ET patients who had received prior treatment with the above drugs (P=0.147).",
}