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Salivary tissue inhibitor of metalloproteinases‐1 localization and glycosylation profile analysis

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Holten‐Andersen L, Thaysen‐Andersen M, Jensen SB, Buchwald C, Højrup P, Offenberg H, Nielsen HJ, Brünner N, Nauntofte B, Reibel J. Salivary tissue inhibitor of metalloproteinases‐1 localization and glycosylation profile analysis. APMIS 2011; 119: 741–9.

Salivary tissue inhibitor of metalloproteinases‐1 (TIMP‐1) has been associated with pathological conditions in the oral cavity, but the origin of TIMP‐1 in saliva remains unknown. Hence, we studied the localization of TIMP‐1 in salivary gland tissue and also investigated if TIMP‐1 found in blood and saliva is identical. Human salivary gland tissue samples (four parotid gland and four submandibular gland biopsies) were analysed for the presence of TIMP‐1 mRNA and protein expression. To assess TIMP‐1 glycosylation profiles in blood and saliva, the protein was isolated from plasma and unstimulated and stimulated whole saliva as well as stimulated parotid and submandibular saliva and analysed by MALDI‐TOF mass spectrometry. TIMP‐1 protein was demonstrated in mucous acinar cells of the submandibular gland and in ductal cells of both the parotid and submandibular gland. However, no TIMP‐1 mRNA was detected in any of these cells. The glycosylation profiles of TIMP‐1 isolated from whole saliva and saliva from the major glands were highly similar. In contrast, a significant difference was found between the glycoprofiles of salivary TIMP‐1 and plasma TIMP‐1. Although no clear evidence of TIMP‐1 transcription in major salivary glands was demonstrated our results suggest that TIMP‐1 in saliva does not originate from plasma.
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Document Type: Research Article

Affiliations: 1: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense 2: Department of Oral Medicine, Clinical Oral Physiology, Oral Pathology & Anatomy, Faculty of Health Sciences, Institute of Odontology, University of Copenhagen, Copenhagen 3: Department of Otolaryngology/Head and Neck Surgery, University Hospital of Copenhagen, Copenhagen 4: Section of Pathobiology, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Copenhagen 5: Department of Surgical Gastroenterology, Hvidovre University Hospital, Hvidovre, Denmark

Publication date: November 1, 2011

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