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Expression of E-cadherin in human mast cell line HMC-1

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Nishida M, Kawai K, Tanaka M, Tegoshi T, Arizono N. Expression of E-cadherin in human mast cell line HMC-1. APMIS 2003;111:1067–74.

E-cadherin is one of the cell adhesion molecules normally expressed on epithelial cells. We previously reported that murine bone marrow-derived mast cells express E-cadherin that could be involved in homophilic binding with epithelial cell E-cadherin. In the present study we examined whether E-cadherin is also expressed in human mast cell HMC-1. Gene expression of E-cadherin and β-catenin was observed in HMC-1 by reverse transcription-polymerase chain reaction (RT-PCR), while N-cadherin expression was undetectable. cDNA sequencing of HMC-1 E-cadherin revealed no deletions or mutations. E-cadherin expression in HMC-1 was confirmed by immunoblotting as well as by flow cytometric analyses. In the presence of E-cadherin blocking antibody or a synthetic E-cadherin decapeptide with HAV sequence in culture medium, adhesion of HMC-1 cells to the A431 epithelial cell monolayer was slightly but significantly suppressed. In contrast, N- or P-cadherin decapeptides did not suppress the binding. These results indicated that human mast cell HMC-1 expresses E-cadherin, and is possibly involved in cellular interactions with epithelial cells, while other functions still remain to be elucidated.
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Keywords: E-cadherin; HMC-1; Human; mast cell; β-catenin

Document Type: Research Article

Affiliations: 1: Research and Development Center, Fuso Pharmaceutical Industries Ltd., Osaka, Japan 2: Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto, Japan

Publication date: November 1, 2003

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