Skip to main content
padlock icon - secure page this page is secure

Interactive functional poly(vinylidene fluoride) membranes with modulated lysozyme affinity: a promising class of new interfaces for contactor crystallizers

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.

BACKGROUND: One of the challenges of current researches in biotechnological fields is the achievement of regular and increasingly smaller protein crystals for genomics and biocatalyst applications. The membrane contactor‐based methodology appears to be a time‐effective and economically competitive technology for accomplishing this target.

RESULTS: A new class of interactive polymeric interfaces enabling the nucleation of very small protein crystals in a short time through controlling attractive interactions is discussed. Specifically, the role of attractive interfacial forces between a model lysozyme solution and modified poly(vinylidene fluoride) membranes is examined. The insertion of amphiphilic motifs in the fluorinated membranes allows quicker agglomeration of protein crystals at the membrane surface reducing significantly the induction time for protein nucleation. The chemical nature of the modifier permits the modulation of the membrane affinity to the lysozyme, involving polar and non‐polar attractive interactions and preserving intrinsic structural features, transport properties and the hydrophobic character of the interfaces, according to the basics of membrane crystallization technology. The formation of critical nuclei is observed after 3 h and micro‐sized crystals are formed in less than 24 h.

CONCLUSION: The experimental evidence suggests these membranes as a promising class of interactive interfaces that may rapidly bring advances in genomics research. Copyright © 2009 Society of Chemical Industry
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: crystals; interfacial interactions; membrane; protein; surface

Document Type: Research Article

Publication date: December 1, 2009

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more