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A new candidate protein for high lysine content in wheat grain

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Lysine is the most limiting essential amino acid in cereal grains, so that grain lysine content is important for human nutrition and livestock growth. Translation elongation factor 1α (EF-1α) from cereal embryo was recently reported to be rich in lysine, and the possibility of using this protein as a marker for feed quality was explored in maize. In this study we used immunochemical methods to investigate the relationship between the content of EF-1α and other proteins from wheat germ and lysine content in both hexaploid (bread) wheats and diploid wheat progenitors to the wheat A-genome. The levels of grain lysine, as well as their variation between lines or cultivars, were greater for the diploid wheats. While there was a significant correlation between the levels of EF-1α and grain lysine content, the binding of antibodies to a protein of Mr 37 000 showed a higher correlation. This protein was characterised by amino acid sequencing as fructose 1,6-bisphosphate aldolase. The possibility of using fructose 1,6-bisphosphate aldolase as a marker for feed quality and development of a simple ELISA for quantification of lysine in wheat is demonstrated.

© 2000 Society of Chemical Industry
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Keywords: aldolase; antibodies; elongation factor 1; lysine; wheat

Document Type: Research Article

Affiliations: 1: CSIRO Plant Industry and Quality Wheat CRC Ltd, GPO Box 1600, Canberra, ACT 2601, Australia 2: University of Sydney and Quality Wheat CRC Ltd, Plant Breeding Institute, PMB 11, Camden, NSW 2570, Australia

Publication date: January 15, 2001

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