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Determination of pK a values of wogonoside,wogonin and oroxylin-A by RP-HPLC

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Objective: To apply the RP-HPLC method for determination of the pK a values of wogonoside,wogonin and oroxylin-A,in order to provide the reference to the rational design of dosage forms and clinical application of the drug. Methods: Acetonitrile-different pH buffer solutions were used as the mobile phase to determinate the K values of wogonoside,wogonin and orgxylin-A on the basis of the relationship of retention factor (K) of the compound investigated and the pH value of the mobile phase.k was determined respectively for wogonoside,wogonin and orgxylin-A and the pK a value was calculated according to the formula of pK a and K. Results: The pH-K plot of wogonoside,wogonin and oroxylin-A was a sigmoid curve and the pK a value was 3.84±0.16,7.48±0.11 and 7.53±0.13 respectively. Conclusion: The proposed method is low sample-consuming,with characteristics of rapid sample analysis,high sensitivity and accuracy,and convenience which could be easily used for analysis of the pK a values of wogonoside,wogonin and oroxylin-A.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: RP-HPLC; dissociation constant of weak acid; oroxylin-A; pKa; physicochemical parameters; wogonin; wogonoside

Document Type: Research Article

Affiliations: 1: College of Chinese Traditional Medicine Guangzhou University of Traditional Chinese Medicine Guangzhou 510006 China 2: School of Chinese Medicine Southern Medical University Guangzhou 510515 China

Publication date: 01 January 2014

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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