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Determination of oleanolic acid and ursolic acid in different parts of Gentiana tizuensis and Gentiana farreri by RP-HPLC

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Objective: To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in different parts of Gentiana tizuensis and Gentiana farreri. Method: The samples were isolated on a Kromasil C18 column(4.6 mm×250 mm,5 μm)with methanol-water containing 0.2% phosphoric acid(90:10)at a flow rate of 0.8 mL·min-1.The detection wavelength was 205 nm and the column temperature was controlled at 30°C. Results: Oleanolic acid and ursolic acid were base-isolated,and the linear ranges were 0.652-6.52 μg(r=0.9998)and 0.996-9.96 μg(r=0.9997),with the average recoveries(n=6)at 101.2%(RSD=1.6%)and 98.0%(RSD=1.7%),respectively. Conclusion: The established RP-HPLC method can simultaneously determine the content of oleanolic acid and ursolic acid in Gentiana tizuensis and Gentiana farreri.The method is convenient,rapid and reliable,which can be applied to the quality control of Gentiana tizuensis and Gentiana farreri.

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Keywords: Gentiana farreri Balf.f; Gentiana tizuensis Franch; HPLC; oleanolic acid; peak purity analysis; ursolic acid

Document Type: Research Article

Affiliations: College of Chemistry and Bioengineering Yichun University Yichun 336000 China

Publication date: January 1, 2013

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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