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Determination of chemical constituents of ether extract of Radix Tetrastigmae Hemsleyani

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To study the chemical constituents of ether extract of Radix Tetrastigmae Hemsleyani.Method:The chemical compositions of the ether extract of the plant were analyzed by GC-MS equipped with an elastic quartz capillary column-HP-Innowax Polyethylene Glycol (30.0 m×320 μm×0.25 μm).The oven temperature program was 50 for 2 min,increased at 4 ·min- to 230 and kept for 20 min.The carrier gas was helium (99.999%) with a flow rate of 2.0 mL·min-.The split ratio was 40:1.MS conditionsion source,EI source;ion source temperature,230 quadrupole pole temperature,150 Mass spectra were taken at 70 eV;emission current,34.6 μAmultiplier voltage,1.936 kVinterface temperature,280 Mass rang was from 10 to 550 amu.The constituents were identified by their mass spectra.The relative percentage of the oil constituents was calculated from the GC peak areas.Result:Twenty-one kinds of chemical constituents were detected in the ether extract of Radix Tetrastigmae Hemsleyani;of which eighteen compounds representing 98.45% of the extract were characterized.Relative contents more than 1% were determined as linoleic acid 35.28%,palmitic acid 26.93%,oleic acid acid 15.56%,diphenylamine 7.40%,methyl linolenate 7.15%,stearic acid 2.14%.Conclusion:The determination of chemical constitutes of ether extract from Radix Tetrastigmae Hemsleyani is helpful for controlling the quality and further researching of Radix Tetrastigmae Hemsleyani.

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Keywords: GC-MS; Radix Tetrastigmae Hemsleyani; ether extract; extract with organic solvent

Document Type: Research Article

Publication date: October 1, 2008

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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