RP-HPLC Studies on Quantitative Determination of Pterostilbene in Dragon′s Blood
To establish a HPLC method for the separation and determination of pterostilbene in Dragon′s Blood.MethodsUsing Phenomenex C18 reverse phase column(250 mm×4.6 mm5 μm)and acetonitrile-1% acetic acid (41:59) as mobile phase,flow rate of 1.5 mL·min-1,detecting
at 310 nm and the sensitivity at 0.02 AUFS,the temperature of column was 40 and quantitating with external standard method.Results:The standard curve of pterostilbene was linear in the concentration range of 41.6208 ng(r=0.999 5).The theoretical plate number was 5 422.The average recovery
was 97.82%.ConclusionThe established method was accurate,sensitive,simple and reliable for determination of pterostilbene in Dragon′s Blood.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: Dragon′s Blood; RP-HPLC; pterostilbene
Document Type: Research Article
Publication date: 01 November 2002
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