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HPLC Determination of Crocin,Crocin and Crocins in Croucus Sativus Extract and Tablets

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This paper reports a reversed phase high performance liquid chromatographic method to determine crocincrocin and crocins in extract and tablets.The experimental conditions of HPLC were as follows:Spherisorb 5 ODS column(150mm×4.6mm)with on-line filter with Guark-column,mobile phase:methanol-water(55:45)detection wavelength 440nm,flow-rate 1.0mL/min,paper feed-rate 0.5cm/min.The recovery of the method:crocin in crocus extracts was 103.3%(RSD3.2%),crocin in crocus extracts was 99.3%(RSD3.7%),crocins in crocus extracts was 100.4%(RSD1.7%)(n=9).Crocin in crocins tablet 98.4%(RSD1.5%),crocin in crocins tablet was 101.7%(RSD1.1%),crocins in crocins tablet was 99.8%(RSD2.0%).The resolution of this method is fine and accurate.It provides a means for the control of crocus extracts and tablets.

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Keywords: Crocus sativus L; HPLC; crocin; crocins tablets

Document Type: Research Article

Publication date: May 1, 1998

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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