Skip to main content
padlock icon - secure page this page is secure

Free Content A case of laboratory cross-contamination of Mycobacterium tuberculosis identified using comparative genomics

Download Article:
(PDF 113.3 kb)
BACKGROUND: Two false-positive tuberculosis (TB) cases in Yamagata Prefecture, Japan, 2016.

OBJECTIVE: To report the effectiveness of comparative genomics of Mycobacterium tuberculosis for identification of cross-contamination cases.

DESIGN: Case report of laboratory cross-contamination.

RESULTS: Beginning with detection of an identical genotype in two M. tuberculosis strains using variable number of tandem repeat typing, we suspected M. tuberculosis cross-contamination of specimens collected in a mycobacteriology laboratory based on epidemiological investigations. This suspicion was confirmed using comparative genomics of the two M. tuberculosis strains and a strain from an epidemiologically unrelated specimen from the same batch as the two strains in the mycobacteriology laboratory. All strains had an identical genomic sequence with no single nucleotide variants.

CONCLUSION: Comparative genomics, which offers the highest discrimination power, is a potent tool for identifying laboratory cross-contamination using epidemiological investigations.
No Reference information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: VNTR typing; acid-fast bacilli smear test; false-positive; side effects; unnecessary treatment

Document Type: Research Article

Affiliations: 1: Yamagata Prefectural Institute of Public Health, Yamagata 2: Nagasaki University, Nagasaki 3: Murayama Public Health Center, Yamagata 4: Okitama Public Health Center, Yamagata 5: Shonai Public Health Center, Yamagata, Japan 6: Yamagata Prefectural Institute of Public Health, Yamagata, Murayama Public Health Center, Yamagata

Publication date: October 1, 2018

This article was made available online on August 2, 2018 as a Fast Track article with title: "A case of laboratory cross-contamination of Mycobacterium tuberculosis identified using comparative genomics".

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease (IJTLD) is for clinical research and epidemiological studies on lung health, including articles on TB, TB-HIV and respiratory diseases such as COVID-19, asthma, COPD, child lung health and the hazards of tobacco and air pollution. Individuals and institutes can subscribe to the IJTLD online or in print – simply email us at [email protected] for details.

    The IJTLD is dedicated to understanding lung disease and to the dissemination of knowledge leading to better lung health. To allow us to share scientific research as rapidly as possible, the IJTLD is fast-tracking the publication of certain articles as preprints prior to their publication. Read fast-track articles.

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more