@article {Zallet:2018:1027-3719:945,
title = "Evaluation of OMNIgene	extregisteredSPUTUM reagent for mycobacterial culture",
journal = "The International Journal of Tuberculosis and Lung Disease",
parent_itemid = "infobike://iuatld/ijtld",
publishercode ="iuatld",
year = "2018",
volume = "22",
number = "8",
publication date ="2018-08-01T00:00:00",
pages = "945-949",
itemtype = "ARTICLE",
issn = "1027-3719",
eissn = "1815-7920",
url = "https://www.ingentaconnect.com/content/iuatld/ijtld/2018/00000022/00000008/art00022",
doi = "doi:10.5588/ijtld.17.0020",
keyword = "TB, mycobacteria, tuberculosis, culture contamination",
author = "Zallet, J. and Olaru, I. D. and Witt, A-K. and Vock, P. and Kalsdorf, B. and Andres, S. and Hillemann, D. and Kranzer, K.",
abstract = "SETTING: National Mycobacterium Reference Laboratory, Borstel, Germany.OBJECTIVE: To evaluate the effectiveness of OMNIgene	extregisteredSPUTUM (OM-S) reagent in comparison with a method using N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) with regard to mycobacterial
recovery and contamination of broth and solid cultures.DESIGN: Sputum samples from patients with tuberculosis and other respiratory diseases underwent decontamination with NALC-NaOH-based (MycoDDR) or OM-S reagent. The decontamination procedure was assigned by block randomisation.
Samples were inoculated on L{\"o}wenstein-Jensen, Stonebrink and MGIT (Mycobacterial Growth Indicator Tubes). Mycobacterial recovery from samples spiked with Mycobacterium tuberculosis following decontamination was determined.RESULTS: Eighty-five samples were randomised
to NALC-NaOH and 84 to OM-S reagent. Mycobacterial recovery was significantly lower for samples processed with OM-S reagent compared with the NALC-NaOH method across all media types. Culture contamination was lower with NALC-NaOH reagent on solid media (9.412.9% vs. 28.629.8%).
Growth was not observed in MGIT among samples spiked with 1060016800 colony-forming units of M. tuberculosis following decontamination with OM-S reagent.CONCLUSION: Low mycobacterial recovery, especially in MGIT, observed in the present study suggests that
OM-S reagent might not be compatible with the MGIT system. More extensive field evaluations of the OM-S reagent are warranted to demonstrate a significant benefit over currently used methods.",
}