@article {Majumdar:2016:1027-3719:166,
title = "Detection of multidrug-resistant tuberculosis using MGITTM and MAS-PCR in Tripura, India",
journal = "The International Journal of Tuberculosis and Lung Disease",
parent_itemid = "infobike://iuatld/ijtld",
publishercode ="iuatld",
year = "2016",
volume = "20",
number = "2",
publication date ="2016-02-01T00:00:00",
pages = "166-169",
itemtype = "ARTICLE",
issn = "1027-3719",
eissn = "1815-7920",
url = "https://www.ingentaconnect.com/content/iuatld/ijtld/2016/00000020/00000002/art00008",
doi = "doi:10.5588/ijtld.14.0986",
keyword = "RNTCP, drug susceptibility testing, primary resistance, designated microscopy centre",
author = "Majumdar, T. and Bhattacharya, S. and Barman, D. and Bhoumik, P. and Bir, R.",
abstract = "BACKGROUND: Multidrug-resistant tuberculosis (MDR-TB) poses a global threat that is further compounded by the human immunodeficiency virus (HIV) epidemic.OBJECTIVE: To detect MDR-TB among pulmonary TB (PTB) patients with or without HIV coinfection by isolating and identifying Mycobacterium
tuberculosis from clinical samples and performing drug susceptibility testing (DST).METHODS: Sputum was collected from presumed PTB cases. Microscopic examination was performed following Ziehl-Neelsen (ZN) staining and cultured in L{\"o}wenstein-Jensen (LJ) medium. First-line anti-tuberculosis
DST of the isolates was performed using MGIT (Mycobacterial Growth Indicator Tube) and multiplex allele-specific polymerase chain reaction (MAS-PCR).RESULTS: Of 172 study subjects, 59.3% (102/172) were smear-positive and 40.7% (70/172) were smear-negative. In the smear-positive
and -negative groups, respectively 62.7% (64/102) and 8.6% (6/70) were culture-positive. DST on MGIT showed a cumulative resistance of 7.1% (5/70) to isoniazid (INH) and rifampicin. More ethambutol (EMB) and combined INH+EMB resistance was detected using MAS-PCR.CONCLUSION: MDR-TB is a
problem in Tripura, and culture and phenotypic DST are required for diagnosis. MAS-PCR may provide an alternative rapid screening tool.",
}