Skip to main content
padlock icon - secure page this page is secure

Free Content Evaluation of new GenoType® MTBDRplus for detection of resistance in cultures and direct specimens of Mycobacterium tuberculosis

Download Article:
 Download
(PDF 267.1 kb)
 
SETTINGS: Molecular methods frequently used in laboratories can now give us useful information about low growth bacteria.

OBJECTIVE: To evaluate the new GenoType® MTBDRplus assay for its ability to detect mutations in the 81-bp hotspot region of the rpoB gene, mutations in codon 315 of the katG gene and alterations in the inhA promoter region.

DESIGN: Prospective resistance to rifampicin (RMP) and isoniazid (INH) study using Mycobacterium tuberculosis positive specimens and cultures comparing the results of GenoType MTBDRplus with those obtained phenotypically with the Bactec MGIT (Mycobacterial Growth Indicator Tube) 960.

RESULTS: In 59 specimens (18 smear microscopy samples and 41 solid and liquid medium cultures), mutations were detected in all of 36 M. tuberculosis strains phenotypically resistant to RMP (100%), and in 35 of 37 strains phenotypically resistant to INH (94.59%). The new assay prompted a 21.6% increase in the direct detection of INH resistance in the strains studied, due to the incorporation of inhA promoter region probes in the test.

CONCLUSIONS: The GenoType MTBDRplus assay is a valid method for detecting the most common mutations in strains resistant to RMP and INH. However, further phenotypic testing is required, as the assay failed to detect 100% of INH and RMP resistance.
No Reference information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: GenoType MTBDRplus; Mycobacterium tuberculosis; isoniazid; resistance; rifampicin

Document Type: Regular Paper

Affiliations: 1: Microbiology Service, Reina Sofia University Hospital, Cordoba, Spain 2: Mycobacteria Reference Centre, Department of Microbiology, Faculty of Medicine, Cordoba University, Cordoba, Spain 3: Microbiology Service, Reina Sofia University Hospital, Cordoba, Spain; and Mycobacteria Reference Centre, Department of Microbiology, Faculty of Medicine, Cordoba University, Cordoba, Spain

Publication date: December 1, 2008

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease (IJTLD) is for clinical research and epidemiological studies on lung health, including articles on TB, TB-HIV and respiratory diseases such as COVID-19, asthma, COPD, child lung health and the hazards of tobacco and air pollution. Individuals and institutes can subscribe to the IJTLD online or in print – simply email us at [email protected] for details.

    The IJTLD is dedicated to understanding lung disease and to the dissemination of knowledge leading to better lung health. To allow us to share scientific research as rapidly as possible, the IJTLD is fast-tracking the publication of certain articles as preprints prior to their publication. Read fast-track articles.

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more