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Spacer oligonucleotide typing of bacteria of the Mycobacterium tuberculosis complex: recommendations for standardised nomenclature [The Language of Our Science]

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Spacer oligonucleotide typing (spoligotyping) is widely used for differentiation of bacteria of the Mycobacterium tuberculosis complex. However, the absence of any standardised method for concise description of spoligotypes makes it difficult to compare the results from different laboratories. This paper describes unambiguous, interconvertible systems for the designation of spoligotype patterns, the adoption of which will be beneficial to mycobacterial research.

Keywords: Mycobacterium tuberculosis; epidemiology; spoligotyping

Document Type: Invited Paper

Affiliations: 1: School of Biological Sciences, University of Surrey, Guildford, Surrey, UK 2: Veterinary Sciences Division, Department of Agriculture and Rural Development, Stormont, Belfast, Northern Ireland 3: Instituto de Biotecnología, CICVyA- INTA, Los Reseros y Las Cabañas, Hurlingham, Argentina 4: Australian Reference Laboratory for Bovine Tuberculosis, Agriculture Western Australia, South Perth, Australia 5: Centers for Disease Control and Prevention, Atlanta, Georgia 6: Wadsworth Center, New York State Department of Health, Albany, New York 7: Rijksinstituut voor Volksgezondheid en Milieuhygiene, Bilthoven, Netherlands 8: International Reference Laboratorium for Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark 9: Molecular Mycobacteriology Laboratory, Department of Clinical Investigation, Texas Center for Infectious Disease, San Antonio, Texas 10: Tuberculosis & Mycobacteria Unit, Institut Pasteur de Guadeloupe, Morne Jolivière, Pointe-à-Pitre, Guadeloupe 11: Laboratoire de Référence des Mycobactéries, Institut Pasteur, Paris, France

Publication date: March 1, 2001

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