Flow cytometry as a tool for analyses of soya bean seed vigour
Vigour tests of soya bean seeds are important in seed production; however, many of them have a number of steps and are time-consuming. The analysis of the cell cycle / endoreduplication by flow cytometry can be an alternative to supplement seed quality analyses. Therefore, the aim of this study was to evaluate the potential of flow cytometry to assess soya bean seed vigour compared with other commonly used vigour tests. Four lots of soya bean seeds were subjected to a standard germination test, accelerated ageing test, electrical conductivity test, seedling emergence in sand, and computerised image analysis of seedlings (SVIS®) Dry seeds and those hydrated for 24 hours were evaluated by flow cytometry to determine cell cycle activity and endoreduplication intensity in different seed parts (embryo axis and cotyledons). Seed lots determined by the conventional tests as having high quality, possessed a greater proportion of cells with the highest DNA contents (4C in the embryo axis and 8C in the cotyledons) than those of lower quality. It is suggested that in these lots activation of mitotic cycle / endoreduplication during imbibition was faster and therefore they expressed higher vigour. In conclusion, flow cytometric analysis of seeds has the potential for a fast and reliable evaluation of soya bean seed vigour.
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Document Type: Research Article
Publication date: 01 July 2018
This article was made available online on 25 April 2018 as a Fast Track article with title: "Flow cytometry as a tool for analyses of soya bean seed vigour".
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