Skip to main content
padlock icon - secure page this page is secure

Germination and cryopreservation responses of Jatropha curcas in relation to seed quality

Buy Article:

$30.00 + tax (Refund Policy)

Ex vitro and in vitro germination in relation to seed quality, cryopreservation and seedling regeneration were examined following liquid nitrogen storage of Jatropha curcas seeds and zygotic embryonic axes. Six seed weight categories were used: (1) ≤0.399 g; (2) 0.400 - 0.499 g; (3) 0.500 - 0.599 g; (4) 0.600 - 0.699 g; (5) 0.700 - 0.799 g; (6) ≥0.800 g. Germination was related to the weight and quality of seeds, the presence / absence of the seed coat and type of explant. The moisture content and water uptake during five hours imbibition were related to the initial fresh weight of seeds. The highest germination (90%) was similar under ex vitro and in vitro conditions for seeds with seed coat in the weight category 0.600 - 0.699 g. Shoot regeneration following liquid nitrogen (-196°C) storage was influenced by the type of explant used. Zygotic embryonic axes excised from cryopreserved seeds displayed the highest regeneration percentages with 95% compared with 64% obtained for isolated embryonic axes cryopreserved in plant vitrification solution droplets. Plants developed from cryopreserved seeds and isolated zygotic embryo axes were morphologically similar to the non-cryopreserved controls.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: 01 December 2014

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more