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Application of ultrathin-layer isoelectric focusing for genetic purity testing of F1 hybrid seeds in napa cabbage

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To evaluate the genetic purity of napa cabbage (Brassica campestris subsp. pekinensis) hybrid seeds in the laboratory, ultrathin-layer isoelectric focusing (UTLIEF) of seed proteins was used. In the pH range of 5-8, one male marker band and two female marker bands were found in the parental lines of 87-114. Based on these bands, the genetic purity of F1 seeds could be easily determined in the laboratory. The genetic purity determined by UTLIEF of proteins was confirmed by a grow-out test. T he study shows that UTLIEF of seed proteins could replace grow-out tests to determine the genetic purity of napa cabbage hybrid seeds.
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Document Type: Research Article

Publication date: April 1, 2013

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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