Skip to main content
padlock icon - secure page this page is secure

Identification of SSR markers for hybridity and seed genetic purity testing in sunflower (Helianthus annuus L.)

Buy Article:

$35.00 + tax (Refund Policy)

Hybrid purity is one of the most important characteristics of good quality seed. In order to identify pure hybrids and pollen shedders/offtypes an investigation was done to identify an ideal SSR marker. 58 primer pairs were screened to identify specific markers associated with each hybrid and parental lines. Hybrid KBSH-44 could be clearly identified by using 'ORS 309 and ORS 170' based on the banding pattern resolved on Polyacrylamide gel (6%). The complementary banding pattern of both parents identified the hybrid. ORS 309 amplified allele size of 250bp was specific to the female parent (CMS-17A) and 230 bp was specific to the male parent (RHA 95-C-1). These two bands of allele size 230 and 250bp were found in hybrid KBSH-44 only. Another SSR primer 'ORS 170' was able to distinguish the hybrid KBSH-44 by amplifying allele of size 230bp a female specific (CMS-17A) allele and 200 bp amplicon a male specific allele (RHA 95-C-1). SSR primer ORS 811 found specific to identify KBSH-53 and it amplified allele of size 270bp in its female parent (CMS-53A) and allele size of 230bp in its pollen parent (RHA 95-C-1). The hybrid has both the alleles from its parents at 270 and 230bp.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: April 1, 2011

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more