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Cryopreservation of seeds and embryonic axes of wild apricot (Prunus armeniaca L.)

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Wild apricot (Prunus armeniaca L.) is an economically important fruit crop with rich germplasm variability existing in the high altitude and cold desert areas of India. Due to the vast economic importance of this fruit species and availability of diverse genetic resources, there is an urgent need to collect, characterize and conserve the existing genetic variability of apricot for safe protection and utilization. A cryopreservation protocol for seeds, half seeds and embryonic axes has been successfully devised using desiccation-followed by fast-freezing with high recovery growth percentages. Whole seeds showed viability in the range of 82.5 to 92.5%, half seeds from 85 to 100% and embryonic axes from 98.5 to 100%. The excised embryonic axes showed the highest values of germination in both unfrozen and frozen samples. A total of 250 accessions belonging to 28 folk cultivars have been successfully cryostored as a base collection in the National Cryogenebank at NBPGR, New Delhi, India. The conserved germplasm represents the invaluable variability existing in both cultivated and wild populations of apricot present in India.

Document Type: Research Article

Publication date: April 1, 2010

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