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Regenerated silk fibroin as an inkjet printable biomaterial

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Regenerated silk fibroin (RSF) protein is an FDA approved biomaterial and has been used as a bio-ink to fabricate structures using inkjet printing. Silk can be present in water soluble amorphous (Silk I) and water insoluble crystalline conformations (Silk II) made up of beta-sheet structures. Here we show the generation of silk scaffolds by inkjet printing of water soluble RSF inks and then converting them into insoluble beta-sheet (Silk II) structure via a second ink containing methanol. This paper focuses on optimising printing conditions of RSF bio-ink through establishing the relationships between RSF peptide concentrations, number of layers and the total thickness of the printed layers. Various patterns such as dot arrays, lines, films, particles and logos have successfully been fabricated. The emerging of inkjet printing of RSF ink allows us to print delicate silk scaffold patterns for different applications specifically in biomedical field.
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Document Type: Research Article

Publication date: September 12, 2016

More about this publication?
  • For more than 30 years, IS&T's series of digital printing conferences have been the leading forum for discussion of advances and new directions in 2D and 3D printing technologies. A comprehensive, industry-wide conference that brings together industry and academia, this meeting includes all aspects of the hardware, materials, software, images, and applications associated with digital printing systems?particularly those involved with additive manufacturing and fabrication?including bio-printing, printed electronics, page-wide, drop-on-demand, desktop and continuous ink jet, toner-based systems, and production digital printing, as well as the engineering capability, optimization, and science involved in these fields. In 2016, the conference changed its name formally to Printing for Fabrication to better reflect the content of the meeting and the evolving technology of printing.

    Please note: For purposes of its Digital Library content, IS&T defines Open Access as papers that will be downloadable in their entirety for free in perpetuity. Copyright restrictions on papers vary; see individual paper for details.

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