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Digital Staining of Pathological Tissue Specimens with PCA-based Feature Extraction and Linear Mapping of Spectral Transmittance

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Color is an important parameter in pathological diagnosis and it is the very reason why tissue samples are stained, since the morphological structure of the tissue components can only be vividly observed when they are colored differently. Common for routine staining is the Hematoxylin and Eosin(HE) dyes, however to assess diseases related to the condition of the fibrosis, Masson-trichrome (MT) dyes are used instead. The digital transformation of an HE-stained image to its MT-stained (digital staining) equivalent has already been proposed, where the information derived from the 16-band images of the stained specimens were utilized. In this paper we addressed the possible reduction of the spectral dimension requirement to implement the proposed digital staining procedure. To find for the effective spectral dimension, from the classification point of view, principal component analysis (PCA) was applied independently to the five statistical descriptors of the tissue components transmittance spectra, i.e. mean, maximum, minimum, range and standard deviation. In our initial experiments with liver tissue specimens, it was found that ten principal components can be effective to implement the digital staining scheme.
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Document Type: Research Article

Publication date: January 1, 2005

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  • CIC is the premier annual technical gathering for scientists, technologists, and engineers working in the areas of color science and systems, and their application to color imaging. Participants represent disciplines ranging from psychophysics, optical physics, image processing, color science to graphic arts, systems engineering, and hardware and software development. While a broad mix of professional interests is the hallmark of these conferences, the focus is color. CICs traditionally offer two days of short courses followed by three days of technical sessions that include three keynotes, an evening lecture, a vibrant interactive (poster) papers session, and workshops. An endearing symbol of the meeting is the Cactus Award, given each year to the author(s) of the best interactive paper; there are also Best Paper and Best Student Paper awards.

    Please note: for Purposes of its Digital Library content, IS&T defines Open Access as papers that will be downloadable in their entirety for free in perpetuity. Copyright restrictions on papers vary; see individual paper for details.

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