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Color Correction of Red Blood Cell Area in H&E Stained Images by Using Multispectral Imaging

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The color of stained pathological images varies depending on staining conditions. Since pathologists based their diagnosis on changes in color and morphology of a particular tissue component, it is important that color variations in stained pathological images be corrected so as to obtain a more reliable diagnosis. A color correction for Hematoxylin & Eosin (H&E) stained images was proposed previously. However, the method did not successfully correct the color of red blood cell (RBC) but only the nucleus and cytoplasm. In this paper, a modified color correction method that can appropriately correct the color of RBC areas in H&E stained images is proposed. In the method, a different weighting coefficient was assigned to RBC areas, which was based from the averaged dye amount ratio in RBC areas in reference and test slides, and a new basis function was derived, in addition to the original three bases functions, to represent the change in the absorption coefficient in RBC areas. In the experiment, the effectiveness of the proposed method was shown with respect to the color difference between reference and test slides in CIE-LAB space.
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Document Type: Research Article

Publication date: January 1, 2008

More about this publication?
  • Started in 2002 and merged with the Color and Imaging Conference (CIC) in 2014, CGIV covered a wide range of topics related to colour and visual information, including color science, computational color, color in computer graphics, color reproduction, volor vision/psychophysics, color image quality, color image processing, and multispectral color science. Drawing papers from researchers, scientists, and engineers worldwide, DGIV offered attendees a unique experience to share with colleagues in industry and academic, and on national and international standards committees. Held every year in Europe, DGIV papers were more academic in their focus and had high student participation rates.

    Please note: For purposes of its Digital Library content, IS&T defines Open Access as papers that will be downloadable in their entirety for free in perpetuity. Copyright restrictions on papers vary; see individual papers for details.

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