Skip to main content
padlock icon - secure page this page is secure

Free Content Sodium Hydrosulfide Exerts a Transitional Attenuating Effect on Spermatozoa Migration in Vitro

Download Article:
(PDF 404.6 kb)
Hydrogen sulfide (H2S) has been shown to have a prominent role in the regulation of reproductive system function and fertility. The aim of the study was to assess the effect of a H2S donor, sodium hydrosulfide (NaHS), on mouse sperm migration in vitro. Special plates with 4 corner wells filled with balanced salt solution (control) and various NaHS solutions in concentrations of 2.5 mmol/l, 5 mmol/l or 10 mmol/l were applied. Spermatozoa from each male mouse were injected (the experiment was repeated with ten BALB/c 5-month old males) into the central pocket, connected with the wells with ducts. After 1 h, 2 h and 4 h of incubation, the number of spermatozoa in each well was determined using B├╝rker's counting chambers. The number of spermatozoa in all corner wells were summed and the number of the cells in each well was treated as the percentage share of all the migrated spermatozoa. At the time points of 1 hour and 4 hours, no differences regarding chemotactic features of spermatozoa to the utilized solutions were observed. After two hours of incubation the attenuating effect of NaHS medium and high level solutions on spermatozoa migration was observed, but not for the low concentration mixture: H(3, N = 40) = 9.65, P = 0.022; control group vs 5 mmol/l NaHS solution: 36.0% vs 18.5%, P = 0.023; control group vs 10 mmol/l NaHS solution group: 36.0% vs 17.0%, P = 0.011. In conclusion, NaHS has a transitional attenuating effect on spermatozoa migration in vitro.

34 References.

No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: 01 April 2015

More about this publication?
  • Folia biologica is an international quarterly journal that publishes papers on the broad field of experimental zoology, nuclear and chromosome research, and also ultrastructural studies. All papers are subject to peer reviews. Indexed in: ISI Master Journal List, Current Contents, Polish Scientific Journals Contents. I.F. 0.667
  • Editorial Board
  • Information for Authors
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more