Skip to main content
padlock icon - secure page this page is secure

Making the first step: practical considerations for the isolation of low-copy nuclear sequence markers

Buy Article:

$14.25 + tax (Refund Policy)

In many plant groups, the use of low-copy nuclear sequence markers for phylogenetics and population genetics has been hindered by their limited availability. Although it may be possible to PCR amplify low-copy markers using primers designed for use with other plant groups, this does not always yield the desired results. Here, we suggest several alternative approaches to begin the isolation and characterisation of novel low-copy markers when there is little or no sequence information available. These alternatives are: (1) the design of new primers from information in the sequence databases; (2) isolation of homologous DNA using a gene probe from another organism; (3) characterisation of sequence markers from DNA fingerprints; and (4) obtaining novel sequences via cDNA cloning.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Affiliations: 1: Department of Systematic and Evolutionary Botany, Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria 2: Department of Evolutionary Biology, Institute of Zoology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria

Publication date: 01 August 2005

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more