Objective: To evaluate phytochemicals and antimicrobial activity of Randia spinosa (Thunb.)
Poir. and Dillenia pentagyna Roxb. leaf extracts against human pathogens such as Gram
positive bacteria (Streptococcus faecalis, Bacillus subtilis and Staphyllococcus aureus), Gram
negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa), and
a fungus Candida albicans.
Methods: Antimicrobial activity of methanol, acetone and ethyl acetate extracts of the two
plants against the human pathogens was investigated by agar well diffusion method, and
qualitative phytochemical screening was conducted for the presence of phytoconstituents such
as alkaloids, catechins, coumarins, flavonoids, phenols, quinones, saponins, steroids, tannins
Results: The qualitative phytochemical analysis showed the presence of diverse range of
compounds like alkaloids, flavonoids, phenols, steroids, tannins, terpenoids and saponins. The
plants exhibited broad spectrum of antimicrobial activities against all the tested bacterial and
fungal pathogens. The maximum zone of inhibition was observed in methanol extracts when
compared with acetone and ethyl acetate extracts. The present study demonstrated that the
selected plants had promising effect on the bacterial and fungal pathogens.
Conclusions: The phytochemicals in the plants may be potentially responsible for the
antimicrobial efficacy of these medicinal plants.
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Document Type: Research Article
Research Department of Botany, V.H.N. Senthikumara Nadar College (an autonomous institution affiliated to Madurai Kamaraj University, Madurai), Virudhunagar 626 001, Tamil Nadu, India
Publication date: January 1, 2016