Ameliorative and antidyslipidemic potentials of aqueous leaf extract of Gongronema latifolium in CCl4 carbon tetrachloride-induced oxidative stress rats
Objective: To investigate the effect of leaf aqueous extract from Gongronema latifolium
(G. latifolium) on CCl4 induced-oxidative stress in Wistar rat. This effect was assessed by
measuring liver marker enzymes activity, analyzing the antioxidant parameters, lipid profile
estimation and lipid peroxidation by-product following CCl4 induced-oxidative stress.
Methods: Milled G. latifolium leaves were subjected to aqueous extraction and the filtrate
was evaporated between 40–60 °C under reduced pressure and a calculated volume of the
leaf extract was administered at a dose of 500 mg/kg body weight. Thirty-five rats were
grouped into seven groups of 5 animals each namely; control and experimental groups. The
experimental groups were treated with 2.0 mL/kg body weight CCl4, 25 mg/kg body weight/
day silymarin (a standard hepatoprotective antioxidant), 500 mg/kg body weight aqueous
extract of G. latifolium leaves were administered to the CCl4 treated rats for 21 days.
Results: Administration of the extract and silymarin increase significantly (P < 0.05) in liver
marker enzyme (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase
and gamma-glutamyl transpeptidase) activities in serum, liver and kidney in the treated groups
when compared with untreated groups. The antioxidant parameters (catalase, peroxidase and
glutathione S-transferase) were significantly elevated (P < 0.05) in animals treated with the extract
and silymarin in comparison with untreated groups. CCl4 induced oxidative stress mediated
variations in total cholesterol, triacylglycerides, high-density lipoprotein cholesterol, low density
lipoprotein cholesterol and very low-density lipoprotein cholesterol were restored significantly (P
< 0.05) by the extract.
Conclusions: The result obtained from this study indicated the antioxidant and antidyslipidemic
potentials of the aqueous extract of G. latifolium leaves.
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Document Type: Research Article
Department of Biological Science, College of Natural Science, Al-Hikmah University, Ilorin, P. M. B. 1601, Kwara State, Nigeria
Department of Biosciences and Biotechnology (Biochemistry Unit), Kwara State University, Malete, P.M.B. 1530, Ilorin, Nigeria
Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex, Sheda, Abuja
Publication date: January 1, 2016