Marine natural product, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- (C7H10N2O2) of antioxidant properties from Bacillus species at Lakshadweep archipelago
Objective: To investigate the antioxidant property of purified bioactive compound from sponge
associated bacteria Bacillus species.
Methods: The potential active compound was subjected for assay of total antioxidant activity,
α,α-diphenyl-α-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide radical
scavenging activity, hydrogen peroxide (H2O2) scavenging activity and total reducing power.
Further, the 16S rRNA gene sequence was carried out to identify the sponge symbiotic bacteria.
Results: The results showed linear increase of total antioxidant activity, DPPH radical scavenging
activity, nitric oxide radical scavenging activity, H2O2 scavenging activity and total reducing
power. IC50 value of the active compound for DPPH activity, H2O2 scavenging activity, nitric oxide
scavenging activity was recorded as 15.025 μg/mL, 23.73 μg/mL, and 41.70 μg/mL respectively. The
potential strain was identified as Bacillus species from GenBank database (GenBank Accession
number JX985748).
Conclusions: The present study has reported the antioxidant property of purified bioactive
compound from sponge associated Bacillus species. The report will be helpful to pharmaceutical
and antioxidant researchers for further studies.
Document Type: Research Article
Affiliations: 1: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai–608502, Tamil Nadu, India; Centre for Marine Living Resources and Ecology-Field Research Station, Agatti Island-682553, Lakshadweep, India 2: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai–608502, Tamil Nadu, India
Publication date: 01 January 2014
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