Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
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Objective: T o detect Botrytis cinerea ( B. cinerea ) latent infections on apples before storage, which
is essential for effective control strategies in the fruit postharvest industry.
Methods: I n the present study, a polymerase chain reaction detection method, based on primers
designed on B. cinerea transposable elements ( boty and flipper ) and intergenic spacer region as
internal control, were utilized to reveal the presence of symptomless infections on apple fruits.
T his molecular method proved to be highly specific and sensitive in detecting latent infections. I t
revealed the presence of the pathogen in 83 % of the samples from infected apples with 10
4 conidia/
m L , whereas those infected with 10
6 conidia/m L detected 94 % as compared to the traditional
method that revealed the pathogen in 40 % and 66 % of the samples inoculated with 10
4 and 10 6
conidia/m L respectively. F urthermore, the method characterized B. cinerea as subpopulation
transposa-type by the presence of the transposable elements boty and flipper
Results: T he results obtained from DNA quantification method were compared with enzyme-
linked immunosorbent assay and these studies showed good correlation. T herefore our method
has important advantages compared with others detection methods for B. cinerea, because
the proposed methodology allowed distinguishes between its two subpopulations ( vacuma and
transposa ) and this would allow establish possible appropriate control strategies.
Conclusions: F inally, the method can be an interesting alternative for its possible application in
the phytosanitary programs of the fruit industry worldwide.
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Document Type: Research Article
Affiliations:
1:
Area of Chemistry and Biotechnology Technology, Department of Chemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Chacabuco 917, D5700BWS San Luis, Argentina
2:
Inquisal-Conicet, Department of Chemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Chacabuco 917, D5700BWS San Luis, Argentina
3:
Laboratory of Plant Virology, EEA Mendoza INTA, San Martín 3853, D5507BWS, Mendoza, Argentina
4:
Area of Chemistry and Biotechnology Technology, Department of Chemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Chacabuco 917, D5700BWS San Luis, Argentina; Inquisal-Conicet, Department of Chemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Chacabuco 917, D5700BWS San Luis, Argentina
Publication date:
01 January 2014