Biochemical changes of Litopenaeus vannamei and Fenneropenaeus indicus in the different stages of WSSV infection
Objective: To find out the difference in the proximate composition and fatty acid profile of
both the species of shrimp Litopenaeus vannamei (L. vannamei) and Fenneropenaeus indicus (F.
indicus) infected with different stages of white spot syndrome virus (WSSV).
Methods: Standard methods were followed by estimating the proximate composition and fatty
acid analysis. Each fish specimens were beheaded, eviscerated and filleted manually. The tissue
samples were oven dried at 67 °C for 24 h. Then the samples were grounded finely with pestle and
mortar. The saponified samples were cooled at room temperature for 25 min. They were acidified
and methylated by adding 2 mL 54% 6 mol/L HCL in 46% aqueous methanol and incubated at 80 °C
for 10 min in water bath. Following the base wash step, the fatty acid methyl esters were cleaned
in anhydrous sodium sulphate and then transferred into gas chromatograph sample vial for
analysis. Fatty acid methyl esters were separated by gas chromatograph.
Results: The proximate composition was higher in the both control tissue than the three (low,
moderate, severe) infected ones. For L. vannamei and F. indicus, the carbohydrates are 5.07% and
6.18%, and the proteins are 25.01% and 22.17%, respectively. Lipid level recorded was little higher
in the shrimps maintained and showed severe sign of WSSV infection than the control and the
fatty acid profile result revealed that saturated fatty acids and monounsaturated fatty acid was in
higher [48.72% (Severe) & 16.87% (low)] L. vannamei. In the polyunsaturated fatty acid, F. indicus
was 40.47% (low).
Conclusions: Our study showed that the healthy shrimps are nutritionally rich than the WSSV
affected shrimps.
Document Type: Research Article
Affiliations: Centre of Advanced Study in Marine Biology, Faculty of Marine Science, Annamalai University, Parangipettai-608 502, Tamilnadu, India
Publication date: 01 January 2013
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