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Open Access Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum

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Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in food safety and controlling the HB. In this study, it was aimed to characterize totally 37 F. culmorum isolates from Turkey via sequence related amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 type assay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 and MAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands, respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were of MAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer sets yielded amplicons from all isolates. Genetic similarity values were ranged from 39 to 94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5 kb. Amplicon numbers for individuals were ranged from 1 to 16. According to data obtained from current study, SRAP based genotyping is powerful tool for supporting the data obtained from investigations including phenotypic and agro-ecological characteristics. Findings showed that SRAP-based markers could be useful in F. culmorum characterization.
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Document Type: Research Article

Affiliations: 1: Department of Molecular Biology and Genetics/Faculty of Arts and Science, Yeni Yüzyil University, 34034 İstanbul 2: Department of Plant Pathology/Faculty of Agriculture, Ondokuz Mayıs University, 55100 Samsun 3: Animal and Plant Production Department/Vocational School, Ondokuz Mayıs University, 55100 Samsun 4: Biotechnology Department/Field Crop Central Research Institute, 06170 Ankara 5: Department of Medical Biology and Genetics/Medical Faculty, Yeni Yüzyıl University, 34034 İstanbul

Publication date: January 1, 2018

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