Phytochemicalassay and Antiplatelet Activity of Fractions of Velvet Bean Seeds (Mucuna pruriens L.)
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Platelet aggregation is an important factor contributing to the formation of thrombus due to an uncontrolled blood clotting. An antiplatelet agent is a compound which decreases platelet aggregation and inhibits thrombus formation. The objectives of this study were to determine the class of compound employing phytochemical assay and to determine the in vitro antiplatelet activity of four fraction, namely hexane, ethyl acetate, butanol, and water fractions of velvet bean seeds (Mucuna pruriens L.) using epinephrine (EPN) as agonist of platelet aggregation. The antiplatelet activities were tested in human platelet rich plasma with hyperaggregation. To determine the activities, EPN was arranged at 4 level of concentrations (300, 150, 75, and 30 ì M), and antiplatelet agents were at 500 µg/ml. The results indicated that ethyl acetate, butanol and water fraction contained high flavonoids and moderate phenols. The water, butanol and ethyl acetate fractions of velvet bean seeds exhibited potential inhibition of EPN-induced platelet aggregation at all concentrations. The strongest antiplatelet agent was water fraction and had the same antiplatelet activity as aspirin at level 150, 75, and 30 ì M of EPN. Butanol fraction had the same antiplatelet activity as aspirin at the lowest EPN (30 ì M).
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Document Type: Research Article
Affiliations:
1:
Department of Chemistry, Faculty of Mathematic and Natural Sciences, University of Jenderal Achmad Yani, Jalan Terusan Jenderal Sudirman, Cimahi 40533, Indonesia
2:
Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jalan Prof. Drg. Suria Sumantri 65, Bandung 40164, Indonesia
3:
Department of Microbiology, Faculty of Medicine, University of Jenderal Achmad Yani, Jalan Terusan Jenderal Sudirman, Cimahi 40533, Indonesia
4:
Department of Agricultural Production, State Polytechnic of Jember, Jalan Mastrip, Jember 68101, Indonesia
5:
Laboratory of Clinical Pathology, Hasan Sadikin Hospital, Jalan Pasteur 38, Bandung 40161, Indonesia
Publication date:
01 January 2010