Microplate Ames MPF™ test use in assessment of mutagenic properties of dust pollution
Background: Highly industrialized Upper Silesia
Region is particularly polluted by both anthropogenic
and natural airborne particulate matters, which may
lead to negative health effects in human.
Materials and methods: The aim of the study was
to assess the mutagenic properties of dust extracts which
were collected in six cities in the Silesian Voivodeship.
Dust samples were collected on glass fiber filters by the
aspirator with air flow ca. 1 m3/min. Extraction of pollution
was carried out using dichlorometane. The
extracted samples were dissolved in dimethylsulfoxide
(DMSO). The mutagenic properties were assessed using
microplate Ames assay MPFTM with the use of bacteria
Salmonella typhimurium strain TA98 and TA100.
Results: In microplate Ames assay MPFTM there was
observed a linear dose-response relationship in both metabolic
variants of TA98 strain. Similar relationship was
observed for TA100 strain with metabolic activation
(S9). Mutagenic activity (AM) of 100% extracts for
TA98 strain in both metabolic variants (S9) exceeded
2, what indicate highly mutagenic effects of dust extracts.
There was no mutagenic activity observed in the assay
with TA100 (S9), AM 1. In the variant with exogenous
metabolic activation (S9) in TA100 strain AM values
ranged from AM1,160,15 to AM9,671,02.
Mutagenic activity varied between different cities.
Conclusions: The study demonstrated that
microplate Ames assay MPFTM is fast and complex
method of assessing the mutagenic properties of dust
pollution, which exert toxic effect on organisms. The
use of microplate Ames assay MPFTM together with
chemical analyses of air dust pollution may evaluate
the level of exposure in the environment and enable to
perform health risk assessment in populations exposed
to mutagenic, toxic and cytotoxic substances.
Document Type: Research Article
Affiliations: Zakład Szkodliwości Chemicznych i Toksykologii Genetycznej, Pracownia Toksykologii Genetycznej, Instytut Medycyny Pracy i Zdrowia Środowiskowego w Sosnowcu
Publication date: 01 January 2012
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