Effects of Astragalus injection on cervical immortalized epithelial cell growth and its cell cycle regulation mechanism
Objective: To study the regulating effect of Astragalus injection on cervical immortalized
epithelial cell growth and cell cycle regulation mechanism. Methods: Cervical immortalized
epithelial cells (H8 cells) were selected for experiment and processed with different
concentrations of Astragalus injection, MTT method was used to detect cell viability, flow
cytometry was used to detect the percentages of different cell cycles and the contents of
different immune cell subsets, and PCR method was used to detect mRNA contents of cell
cycle-related proteins. Results: Astragalus injection could reduce cell viability in a dosedependent
manner, and the inhibiting effect of 200 μg/mL concentration was the most
significant; NK cell and CD3+CD4+CD8-T cell contents as well as CD3+CD4+CD8-/CD3+CD4-
CD8+T cell percentage of 200 μg/mL Astragalus–processing group was higher than those of 0
μg/mL Astragalus–processing group, and CD3+CD4-CD8+T cell content was lower than that of
0 μg/mL Astragalus–processing group; percentages of G0/G1 phase and S phase cells as well as
mRNA contents of cyclinA, cyclinB and cyclinD1 of 200 μg/mL Astragalus–processing group
were lower than those of 0 μg/mL Astragalus–processing group, and percentage of G2/M phase
cell was higher than that of 0 μg/mL Astragalus–processing group. Conclusion: Astragalus
injection has inhibiting effect on the growth of cervical immortalized epithelial cells, and its
mechanisms include regulating immune cell contents, arresting cell cycle and down-regulating
cell cycle-related protein expression.
Document Type: Research Article
Affiliations: 1: LDR Section, International Medical Department, Gansu Provincial Maternity and Child-care Hospital, Lanzhou City, Gansu Province, 730050 2: Lanzhou 504 Hospital of CNNC, Lanzhou City, Gansu Province, 730065 3: Gansu College Traditional Chinese Medicine, Lanzhou City, Gansu Province, 730000
Publication date: January 1, 2016
- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content