Skip to main content
padlock icon - secure page this page is secure

Open Access Effects of Astragalus injection on cervical immortalized epithelial cell growth and its cell cycle regulation mechanism

Download Article:

The full text article is available externally.

The article you have requested is supplied via the DOAJ. View from original source.

This article is Open Access under the terms of the Creative Commons CC BY-NC-ND licence.

Objective: To study the regulating effect of Astragalus injection on cervical immortalized epithelial cell growth and cell cycle regulation mechanism. Methods: Cervical immortalized epithelial cells (H8 cells) were selected for experiment and processed with different concentrations of Astragalus injection, MTT method was used to detect cell viability, flow cytometry was used to detect the percentages of different cell cycles and the contents of different immune cell subsets, and PCR method was used to detect mRNA contents of cell cycle-related proteins. Results: Astragalus injection could reduce cell viability in a dosedependent manner, and the inhibiting effect of 200 μg/mL concentration was the most significant; NK cell and CD3+CD4+CD8-T cell contents as well as CD3+CD4+CD8-/CD3+CD4- CD8+T cell percentage of 200 μg/mL Astragalus–processing group was higher than those of 0 μg/mL Astragalus–processing group, and CD3+CD4-CD8+T cell content was lower than that of 0 μg/mL Astragalus–processing group; percentages of G0/G1 phase and S phase cells as well as mRNA contents of cyclinA, cyclinB and cyclinD1 of 200 μg/mL Astragalus–processing group were lower than those of 0 μg/mL Astragalus–processing group, and percentage of G2/M phase cell was higher than that of 0 μg/mL Astragalus–processing group. Conclusion: Astragalus injection has inhibiting effect on the growth of cervical immortalized epithelial cells, and its mechanisms include regulating immune cell contents, arresting cell cycle and down-regulating cell cycle-related protein expression.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: LDR Section, International Medical Department, Gansu Provincial Maternity and Child-care Hospital, Lanzhou City, Gansu Province, 730050 2: Lanzhou 504 Hospital of CNNC, Lanzhou City, Gansu Province, 730065 3: Gansu College Traditional Chinese Medicine, Lanzhou City, Gansu Province, 730000

Publication date: 01 January 2016

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more