@article {Jaqueline:2008:0103-9016:340,
title = "Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran Atividade da enzima acetilcolinesterase em crebro e msculo de carpas aps exposio aguda ao diafuran",
journal = "Scientia Agricola",
parent_itemid = "infobike://doaj/01039016",
publishercode ="doaj",
year = "2008",
volume = "65",
number = "4",
publication date ="2008-01-01T00:00:00",
pages = "340-345",
itemtype = "ARTICLE",
issn = "0103-9016",
url = "https://www.ingentaconnect.com/content/doaj/01039016/2008/00000065/00000004/art00003",
doi = "doi:10.1590/S0103-90162008000400003",
author = "Jaqueline Ineu Golombieski and Enio Marchesan and Edinalvo Rabaioli Camargo and Jose{\^a}nia Salbego and Joele Schmitt Baumart and Vania Lucia Loro and S{\’e}rgio Luiz de Oliveira Machado and Renato Zanella and Bernardo Baldisserotto",
abstract = "Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term)&58; common carp&58; control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1&59; grass carp&58; control, 1.0, 2.0, 3.0 and 3.5 mg L-1 and, bighead carp&58; control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L-1, as well as the determination of AChE at concentrations near LC50 for these species. LC50 values (nominal concentrations) were 1.81 mg L-1 for the common carp, 2.71 mg L-1 for the grass carp and, 2.37 mg L-1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations) or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (&126;38%) and muscle (&126;50%) of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 mol min-1 g protein-1, respectively). Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide.&60;br&62;Exposio a inseticidas em concentraes elevadas no ambiente podem ocasionar efeitos adversos subletais em organismos aquticos. Alevinos de carpa hngara (Cyprinus carpio, Linnaeus, 1758), carpa capim (Ctenopharyngodon idella, Valenciennes, 1844) e carpa cabea grande (Aristichthys nobilis, Richardson, 1845) foram expostos ao diafuran, um inseticida utilizado na cultura do arroz no sul do Brasil. O objetivo deste estudo foi verificar a relao entre concentrao letal mediana (CL50) do diafuran e a atividade da enzima acetilcolinesterase (AChE) em crebro e msculo dessas espcies, como um possvel biomarcador inicial da exposio a este inseticida. A CL50 foi determinada com peixes expostos a concentraes de diafuran em 96 h&58; carpa hngara&58; controle&59; 0,5&59; 1,0&59; 1,5&59; 2,0&59; 2,5 e 3,0 mg L-1&59; carpa capim&58; controle&59; 1,0&59; 2,0&59; 3,0 e 3,5 mg L-1 e carpa cabea grande&58; controle&59; 0,5&59; 1,0&59; 1,5&59; 2,0&59; 3,0 e 4,0 mg L-1, bem como a determinao da AChE em concentraes prximas da CL50 para essas espcies. Valores de CL50 (concentraes nominais) foram de 1,81 mg L-1 para carpa hngara, 2,71 mg L-1 para carpa capim e 2,37 mg L-1 para carpa cabea grande. Todas as carpas expostas ao diafuran estavam letrgicas (menores concentraes) ou imveis. Diafuran inibiu significativamente a atividade da AChE em crebro (&126;38 %) e msculo (&126;50 %) de todas as espcies estudadas. Atividade da AChE em msculo para carpa cabea grande foi mais alta que crebro (14,44 contra 5,94 mol min-1 g protena-1, respectivamente). Este estudo demonstrou que concentraes de diafuran utilizadas na cultura do arroz podem afetar o comportamento de Cyprinus carpio, Ctenopharyngodon idella e Aristichthys nobilis, e a atividade da acetilcolinesterase cerebral e muscular pode ser um biomarcador inicial de toxicidade deste inseticida.",
}