Nitrogen fixation and growth response of Alnus rubra amended with low and high metal content biosolids Crescimento e fixação de nitrogênio por Alnus rubra cultivado sob fertilização com biosólidos com altos e baixos teores de metais

Forest application of biosolids offers a potential environmentally friendly alternative to landfilling. This two-year investigation was designed to analyze the effects of elevated soil metal concentration resulting from the land application of biosolids on the symbiotic, nitrogen (N) fixing relationship between Alnus rubra Bong. (red alder) and Frankia. High metal biosolids and a modern-day composted biosolid applied at high loading rates of 250, 500, and 1000 Mg ha-1, were used to represent a worst-case scenario for metal contamination. The high metal biosolids were obtained before the current regulations were formulated and had been lagooned prior to use in this study. Total cadmium (Cd), lead (Pb) and zinc (Zn) in the high metal biosolids were 45, 958, and 2623 mg kg-1 respectively. These metal concentrations are above current regulatory limits in the US. The compost was made using biosolids that are currently produced and had Cd, Pb and Zn of 0.8, 20 and 160 mg kg-1 respectively. Trees were harvested and analyzed for rate of N fixation (as measured by acetylene reduction activity), biomass, and foliar metals. Soils were analyzed for available N, total carbon and N, pH and total Cd, Pb and Zn. Rates of N fixation were not affected by soil amendment. In year 2, shoot biomass of trees grown in both the compost and high metal amendments were higher than the control. Shoot biomass increased with increasing amount of compost amendments, but decreased with increasing amount of high metal amendments. There was no relationship between soil metal concentration and plant biomass. Foliar Cd and Pb were below detection for all trees and foliar Zn increased with increasing amount of both compost and high metal amendment, with concentrations of 249 mg kg-1 for trees grown in the compost amendment and 279 mg kg-1 for the high metal amendment. The results from this study indicate that the growth of A. rubra benefited from both types of biosolids used in the study and that the Alnus/Frankia relationship was not negatively impacted by metal concentrations resulting from the high metal biosolids amendments.&60;br&62;A aplicação de biosólidos em florestas representa uma potencial alternativa ambientalmente correta para aterros sanitários. Os efeitos de fertilização de nitrogênio na relação simbiôntica e de fixação de nitrogênio Alnus rubra Bong. (amieiro vermelho) e Frankia foram avaliados em um estudo de campo conduzido por dois anos. Um biosólido com concentração de metais muito acima dos marcos regulatórios (45 mg kg-1 Cd&59; 958 mg kg-1 Pb&59; 2623 mg kg-1 Zn) produzido há mais de 25 anos e um biosólido composto recente foram aplicados nas doses de 250, 500 e 1000 Mg ha-1, representando um cenário pior possível de concentração de metais. Ao final do segundo ano, a biomassa da parte aérea das plantas cultivadas sob ambos os compostos era maior que a das plantas controle, mas não foi observada inter-relação entre a concentração de metais no solo e a biomassa das plantas. As concentrações de Cd e Pb foliares permaneceram abaixo do nível de detecção em todas as plantas, mas a concentração de Zn foliar aumentou proporcionalmente ao aumento da adição do bioremediador, atingindo concentrações de 249 mg kg-1 e 279 mg kg-1 para os tratamentos alto metal e composto, respectivamente. Embora não tenham sido registrados efeitos negativos do biosólido com altos teores de metais, outras rotas de exposição podem levar a possíveis impactos ecológicos.