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Open Access Seleção in vitro de gemas de bananeira 'Nanicão' tolerantes à salinidade In vitro selection of salt tolerant 'Nanicão' banana buds

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O objetivo deste trabalho foi avaliar o efeito do cloreto do sódio (NaCl) sobre o cultivo in vitro de gemas de bananeira cv. Nanicão (AAA) e selecionar as que se mostrassem tolerantes ao estresse imposto. As gemas foram cultivadas em meio nutritivo de Murashige e Skoog (MS) com 2,5 mg L-1 de BAP e suplementado com diferentes níveis de NaCl (0, 20, 40, 60, 80, 100 e 120 mM) durante 60 dias (Fase I). As gemas sobreviventes foram transferidas para o mesmo meio nutritivo MS, sem NaCl, e cultivadas durante 30 dias (Fase II). Em seguida, as gemas foram submetidas, durante 90 dias, aos tratamentos com 80 e 100 mM NaCl (Fase III). Foram selecionadas 100 gemas nessa última fase as quais, logo em seguida, foram colocadas em meio nutritivo sem NaCl, suplementado com 0,5 mg L-1 de ANA e 1,0 mg L-1 de BAP para induzir o enraizamento. As gemas submetidas à concentração de 120 mM de NaCl na Fase I, não sobreviveram ao tratamento. A regeneração de plantas foi fortemente inibida em todas as concentrações com NaCl. Por outro lado, as concentrações de 20 a 80 mM de NaCl provocaram um aumento generalizado no número e tamanho das gemas. Das cem gemas selecionadas a partir dos tratamentos de 80 e 100 mM de NaCl foram regeneradas 80 plantas em meio sem NaCl. O enraizamento não foi significativamente afetado pelo pré-tratamento salino.&60;br&62;This work aims to evaluate the effect of NaCl and to make selections of banana cv. Nanicão (AAA) in vitro. Buds were grown on MS medium with 2.5 mg L-1 BAP and supplemented with different NaCl concentrations (0&59; 20&59; 40&59; 60&59; 80&59; 100 and 120 mM), for 60 days (Phase I). Survivor buds were transferred to salt-free media and grown for 30 days (Phase II). Afterwards they were submitted to MS media containing 80 or 100 mM NaCl, during 90 days (Phase III). One hundred buds were selected from the last phase and then, placed on NaCl-free medium supplemented with 0.5 mg L-1 NAA and 1.0 mg L-1 BAP for rooting. All buds died when submitted to 120 mM NaCl. Plant regeneration was highly inhibited by all NaCl concentrations. On the other hand, concentrations from 20 to 80 mM NaCl increased bud number and length. Buds were selected from treatments with 80 and 100 mM NaCl, and 80 plants were regenerated in media without NaCl. Rooting was not affected by saline pre-treatment.
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Document Type: Research Article

Publication date: January 1, 2000

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