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Micropropagation and cryopreservation of the rare endemic Colchicum figlalii germplasm

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BACKGROUND: The natural population of Colchicum figlalii (Varol) Parolly & Eren grows in a narrow area of serpentine rock clearings at an altitude of 1900-2100 m in Southwestern Anatolia (Sandras Mountain, Mugla, Turkey). The species is regarded as endangered according to the IUCN Red List Categories. OBJECTIVE: To develop an optimum procedure for in vitro propagation and cryopreservation of germplasm of this rare endemic. MATERIALS AND METHODS: A total of 281 bulbs were used as in vitro culture starting material and after surface sterilization, clean material was obtained from 157 of them. Woody Plant Medium (WPM), Olive Medium (OM), and Murashige and Skoog medium (MS) were used for in vitro culture establishment. RESULTS: The maximum regeneration rate (˜67.3%) was obtained after four weeks of incubation on OM. The calli were successfully induced by using OM supplemented with 10.7 μM NAA from leaves of in vitro grown C. figlalii bulbs. A PVS2-vitrification procedure was used for cryopreservation of C. figlalii callus tissue. After cryo-storage, the best result for regeneration (66.7%) was obtained from calli treated with PVS2 for 75 min before plunging into liquid nitrogen. All rooted seedlings derived from cryopreserved calli were successfully acclimatized to greenhouse conditions. CONCLUSION: This study is an effective reference for future long-term conservation of similar species that are difficult to cryopreserve.

Keywords: COLCHICUM FIGLALII; CRYOPRESERVATION; DMSO; PVS2; SOMATIC EMBRYOGENESIS; VITRIFICATION

Document Type: Research Article

Affiliations: 1: Muğla S??tk?? Ko??man University, Faculty of Science, Molecular Biology and Genetics Department, 48000, Muğla, Turkey 2: Eskişehir Osmangazi Univerity, Special Education Application and Research Center, 26040 Odunpazar??-Eski??ehir, Turkey 3: Erciyes University, Seyrani Faculty of Agriculture, Department of Horticulture, 38030, MelikgaziKayseri, Turkey 4: Osmaniye Korkut Ata University, Kadirli School of Applied Sciences, 80760, Kadirli-Osmaniye, Turkey 5: Çukurova University, Faculty of Agriculture, Department of Horticulture, 01250 Sar????am-Adana, Turkey

Publication date: July 1, 2024

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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