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Optimizing semen cryopreservation in Calomys laucha: a step forward in rodent reproductive research

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BACKGROUND: Examining semen cryopreservation in Calomys laucha offers valuable insights for reproductive research and species conservation. OBJECTIVE: To determine the most effective sugar for the cryopreservation of C. laucha semen. MATERIALS AND METHODS: Using 36 epididymides from C. laucha, semen samples were diluted in a 3% skimmed milk medium supplemented with one of four sugars (glucose, fructose, lactose, or sucrose) at a concentration of 0.3 M. These mixtures underwent a conditioning phase at 37°C for 10 min, cooled to −80°C for another 10 min, and were subsequently stored in liquid nitrogen. RESULTS: Upon thawing, samples treated with lactose and glucose solutions show superior sperm motility, achieving 8.2% and 10.0% respectively, in contrast to the fructose (2.0%) and sucrose (4.1%) mixtures. Furthermore, samples preserved in glucose registered the highest sperm penetration rates, reaching 44.9%. CONCLUSION: Our findings suggest that a cryopreservation medium containing 0.3 M glucose can contribute to the safeguarding C. laucha rodent semen.

Keywords: CRYOPRESERVATION; CRYOPROTECTANTS; RODENT; SPERM QUALITY

Document Type: Research Article

Affiliations: 1: Center for Research and Education in Animal Reproduction, Federal University of Pelotas, Faculdade de Veterinária, Campus Universitário, S/N - CEP 96160-000, Capão do Leão, RS - Brasil 2: Université Catholique de Louvain, Université Catholique de Louvain - Bruxelles, Pôle de Recherche en Gynecologie - Bruxelles, Bélgica 3: Center for Research and Education in Animal Reproduction, Federal University of Pelotas, Faculdade de Veterinária, Campus Universitário, S/N - CEP 96160-000, Capão do Leão, RS - Brasi 4: Institute of Biological Sciences, Federal University of Rio Grande, Campus Carreiros: Av. Itália km 8 Bairro Carreiros- CEP 96203-900 Rio Grande, RS, Brazil

Publication date: March 1, 2024

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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