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Role of Oleic Acid and Trehalose on Frozen-Thawed Ram Semen

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BACKGROUND: When sperm are cryopreserved, reactive oxygen species (ROS) are formed that are detrimental to the sperm. OBJECTIVE: To evaluate the effects of oleic acid and trehalose added to ram semen extender on sperm parameters, lipid peroxidation (MDA), and superoxide dismutase (SOD) enzyme levels of spermatozoa following the freeze/thawing processes. MATERIALS AND METHODS: Ejaculates were collected from four rams and pooled at 35°C. Pooled ejaculates were diluted with oleic acid at 0 mM and trehalose at 0 mM (O0 T0) as the control. The Tris-based extender was supplemented with either 0.5 (O0.5) or 1 (O1) mM of oleic acid or 25 (T25) or 50 (T50) mM of trehalose alone, and in combination [0.5 mM oleic acid + 25 mM trehalose (O0.5T25), 0.5 mM oleic acid + 50 mM trehalose (O0.5T50), 1 mM oleic acid + 25 mM trehalose (O1T25) and 1 mM oleic acid + 50 mM trehalose (O1T50)]. The semen was frozen by the traditional liquid nitrogen vapour method and stored at –196°C in the liquid nitrogen tank. RESULTS:Semen extender containing O1T25 significantly improved the total motility, when compared with other treatment groups (P < 0.05), except for O1T50. O1T50 had a higher viability rate than any other treatment. The addition of O1T25 and O1T50 increased DNA and membrane integrity of spermatozoa post-thawing compared to other treatments (P<0.05). The level of MDA was significantly (P<0.05) lower in extenders supplemented with O1, O0.5T25, O0.5T50, O1T25 and O1T50 compared to the other treatment groups. In addition, SOD levels were higher in groups treated with O1T25 and O1T50 than the other treatment groups (P<0.05). CONCLUSION: The addition of a combination of oleic acid and trehalose concentrations to Tris-based extender improved the quality of ram semen post-thawing.

Keywords: FREEZING; OLEIC ACID; SHEEP; SPERMATOZOA; TREHALOSE

Document Type: Research Article

Affiliations: Department of Animal Science, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah, Iran

Publication date: November 1, 2023

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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