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Home / Cryoletters, Volume 44, Number 4

Freezing of Equine Semen is Influenced by Exposure Time and Concentration of the Cryoprotectant Glycerol

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BACKGROUND: Glycerol is a cryoprotectant widely used in the freezing of mammalian species ' semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species. OBJECTIVE: To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen. MATERIALS AND METHODS: The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times. The thawed sperm was evaluated for kinetic parameters using a Computer Assisted Semen Analysis (CASA) system and cell feature parameters were assessed by flow cytometry. RESULTS: Considering the total and progressive motility of the spermatozoa, we concluded that protocols using 5% glycerol for 15 and 30 min exposure, 4% glycerol for 45 min exposure and 3% glycerol for 90 min exposure generated the best results. CONCLUSION: We suggest the use of any of these protocols for a better cryopreservation of equine semen.

Keywords: CONSERVATION; EQUINE; MOTILITY; SPERM

Document Type: Research Article

Affiliations: 1: Programa de pós graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil 2: Reprodução Animal Comparada- RAC, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil; ReproPel, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil 3: Reprodução Animal Comparada- RAC, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil; ReproPel, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil

Publication date: July 1, 2023

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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