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Aqueous Extract of Moringa Oleifera Lam. Leaves Added to Freezing Extenders Damages Goat Sperm Membranes

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BACKGROUND: Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant . OBJECTIVE: The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency. MATERIALS AND METHODS: Ejaculates (n=6) from four goat breeders were pooled and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed. RESULTS: With the SM extender, straightness, wobble and plasma membrane integrity were lower than in the control group (ρ < 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (ρ < 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used, damaged the membrane of sperm cells in a dose-dependent manner. CONCLUSION: The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner.

Keywords: ANTIOXIDANT; NATURAL EXTRACT; REACTIVE OXYGEN SPECIES; SKIMMED MILK; TRIS-EGG YOLK

Document Type: Research Article

Affiliations: 1: Laboratório de Andrologia, Departamento de Reprodução Animal, Universidade Federal Rural de Pernambuco. Recife, Brasil 2: Laboratório de Bioquímica de Prote??nas, Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brasil 3: Laboratório de Microbiologia e Biologia Celular, Departamento de Microbiologia, Instituto Aggeu Magalhaes - Fiocruz, Recife, Brasil

Publication date: May 1, 2023

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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