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Can Honey Improve the Quality of Cryopreserved Cross Bred Ram Semen Added to Tris Egg Yolk Extender?

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Authors: Khatun, Arjuma 1 ; Fazili, M.R. 2 ; Malik, A.A. 1 ; Naikoo, M. 1 ; Choudhury, A.R. 3 ; Shah, Syed 4 ; Lone, F.A. 1 ; Qureshi, S. 5 ; Hussain, I. 5 ; 

Source: Cryoletters, Volume 43, Number 6, November 2022, pp. 334-340(7)

Publisher: Cryoletters

DOI: https://doi.org/10.54680/fr22610110212

BACKGROUND: Honey can improve the quality of cryopreserved ram semen because of its multinutrient and cryoprotective nature added to standard tris egg yolk extender. OBJECTIVE: Different concentrations of honey were added to the standard tris egg yolk extender to improve the post-thaw quality of crossbred ram semen. METHOD: Thirty six (36) ejaculates from eight healthy cross bred rams were pooled and divided into four aliquots. Standard tris egg yolk extender without any alteration acted as Control (C) and was supplemented with different concentrations of honey, viz. T1 (honey 1.5%), T2 (2.5%), and T3 (3.5%). RESULTS: The percent (mean ± S. E. M) sperm motility at pre-freeze was significantly (P<;0.05) higher in Group T2 and at post-thaw in Group T3 in comparison to T1 and C treatment groups. The percent (mean ± S. E. M) HOST reacted spermatozoa at post-thaw was significantly (P< 0.05) higher in Group C and at pre-freeze the value was significantly (P< 0.05) higher in the same treatment group than Group T1. The mean MDA level (mean ± S. E. M) at post thaw was significantly (P<0.05) low er in Group T3 than the treatment groups C and Group T1. CONCLUSION: From this study it is concluded that the addition of 3.5% honey to the standard tris egg yolk extender provides better protection to ram semen than the addition of 1.5% honey (i. e., Control).

Keywords: CRYOPRESERVATION; HONEY; IVF; RAM; SEMEN; TRIS EGG YOLK

Document Type: Research Article

Affiliations: 1: Division of Animal Reproduction, Gynaecology and Obstetrics 2: Mountain Livestock Research Institute 3: Division of Veterinary Anatomy and Histology 4: Frozen Semen Station 5: Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Shuhama, Alusteng, Srinagar - 190006, J & K, India

Publication date: November 1, 2022

More about this publication?

  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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