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Home / Cryoletters, Volume 43, Number 3

Vitrification by Transient Vacuum Flashing Spray Cooling of Liquid Nitrogen

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Authors: Su, Fengmin; Fan, Yiming; Zhang, Chi; Wang, Yifan; Wang, Yanyang; Peng, Benli

Source: Cryoletters, Volume 43, Number 3, May 2022, pp. 167-174(8)

Publisher: Cryoletters

DOI: https://doi.org/10.54680/fr22310110212

BACKGROUND: The transient vacuum flashing spray cooling of liquid nitrogen (LN2 ) on a microstructured surface can provide ultra-fast cooling rate and may improve cell survival rates. OBJECTIVE: To utilize flashing spray cooling of LN2 instead of film boiling to improve further cell vitrification. METHOD: This study analyzed the effects of the three key parameters (flow rate of liquid nitrogen, ambient pressure, and spray distance) on the cooling process by experimentation. RESULTS: The experimental results showed that the vacuum flashing spray cooling of LN2 can gain higher cooling rates than that achieved by film boiling in conventional vitrification methods. The three parameters all affected the vacuum flash evaporation spray cooling of LN2, and their effect trends were not monotonous but followed a parabolic trend that increased and then decreased. That is, the three parameters all have optimum values to the cooling process. CONCLUSION: Vacuum flash evaporation spray cooling can develop the ultra-fast cooling rates needed to enhance cell vitrification.

Keywords: CELL CRYOPRESERVATION; FLASHING SPRAY COOLING; LIQUID NITROGEN; TRANSIENT HEAT TRANSFER

Document Type: Regular Paper

Affiliations: Institute of Marine Engineering and Thermal Science, Dalian Maritime University, Dalian, China

Publication date: May 1, 2022

More about this publication?

  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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