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Cryopreservation Of Pyramimonas Mucifera

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BACKGROUND: It is important to appreciate microalgal diversity, better understand their ecosystem functioning and therefore implement conservation measures. The National Biodiversity Act of South Africa has a marine and coastal component which promotes such investigations. OBJECTIVE: To develop a cryostorage method for the marine unicellular algal species Pyramimonas mucifera. MATERIALS AND METHODS: Cell viability, measured by propidium iodide, was used to determine both optimal exposure time to 10 % DMSO and survival following thawing of cryopreserved cells. Cryopreservation was achieved by a two-step cooling method. RESULTS & DISCUSSION: A 30-min DMSO exposure was selected for P. mucifera, as cells following such treatment retained cell shape and integrity. Although density was significantly reduced after cryopreservation, the surviving cells were capable of returning to viability levels equal to those of the untreated control (>90%). CONCLUSION: Cultures of P. mucifera can be successfully cryopreserved and propidium iodide provides a useful indication of culture vitality.

Keywords: DIMETHYL SULPHOXIDE; PROPIDIUM IODIDE; PYRAMIMONAS MUCIFERA; TWO-STEP COOLING METHOD

Document Type: Research Article

Affiliations: School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, Private Bag 3, Wits, 2050, South Africa

Publication date: January 1, 2022

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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