Skip to main content
padlock icon - secure page this page is secure

A Microfluidic Approach for Cryoprotectant Screening: Preliminary Validation with Human Red Blood Cells

Buy Article:

$22.35 + tax (Refund Policy)

BACKGROUND: The application of microfluidics has overcome a series of long-term existing bottlenecks in the field of cryobiology. However, there is still no investigation regarding the on-chip rapid screening of cryoprotectant concentration optimization for the integration of the entire freeze, thaw and cell counting process. OBJECTIVE: To establish an on-chip approach for rapid cryoprotectant screening and concentration optimization. MATERIALS AND METHODS: Rapid freezing of red blood cells with glycerol and dimethyl sulfoxide was used to validate the proposed onchip method. RESULTS: The optimal cryoprotectant and its concentration can be determined with the described method, without the need of additional samplings and assays. The optimal concentration for glycerol and dimethyl sulfoxide used in the validation experiments was 10% (w/v). CONCLUSION: The microfluidic approach can be used for rapid cryoprotectant screening and concentration optimization.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: CRYOPRESERVATION; CRYOPROTECTANT SCREENING; MICROFLUIDICS

Document Type: Research Article

Publication date: September 1, 2020

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more