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A Microfluidic Approach for Cryoprotectant Screening: Preliminary Validation with Human Red Blood Cells

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BACKGROUND: The application of microfluidics has overcome a series of long-term existing bottlenecks in the field of cryobiology. However, there is still no investigation regarding the on-chip rapid screening of cryoprotectant concentration optimization for the integration of the entire freeze, thaw and cell counting process. OBJECTIVE: To establish an on-chip approach for rapid cryoprotectant screening and concentration optimization. MATERIALS AND METHODS: Rapid freezing of red blood cells with glycerol and dimethyl sulfoxide was used to validate the proposed onchip method. RESULTS: The optimal cryoprotectant and its concentration can be determined with the described method, without the need of additional samplings and assays. The optimal concentration for glycerol and dimethyl sulfoxide used in the validation experiments was 10% (w/v). CONCLUSION: The microfluidic approach can be used for rapid cryoprotectant screening and concentration optimization.
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Document Type: Research Article

Publication date: September 1, 2020

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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